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Picking up three weeks after the events in Resident Evil: Retribution, Alice (Milla Jovovich) awakens in the now-ruined White House, after being betrayed once again by Albert Wesker (Shawn Roberts). While searching for survivors, The Red Queen (Ever Gabo Anderson) appears and tells Alice that she must return to The Hive in Raccoon City within 48 hours, where the Umbrella Corporation has developed an airborne antivirus, which will kill every organism infected by the T-virus, before all humankind will be lost. When asked why she betrayed her creators, the Red Queen simply says that she will tell Alice once she arrives at The Hive. While on her way to Raccoon City, she is captured by Dr. Alexander Isaacs (Iain Glen), long thought to be dead. Isaacs tells her that the one she killed was a clone. He decides toss her to a horde of zombies on an armored vehicle, but Alice escapes through a motorcycle carried within it. Alice later arrives in the now-destroyed Raccoon City. While exploring the streets, she is knocked unconscious and captured by a group of survivors, consisting of Doc (Eoin Macken), Abigail (Ruby Rose), Christian (William Levy), Cobalt (Rola), Razor (Fraser James), and Claire Redfield (Ali Larter), who is revealed to be alive after the attack on Arcadia. Revealing that a group of armored vehicles and a horde of zombies is coming their way, the group prepares to make their last stand. With the help of Abigail, they manage to defeat the enemy forces, although Cobalt is killed in the process. The group later realizes that a second horde is coming to their direction.